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. 2014 Nov 13;9(4):1202–1208. doi: 10.1016/j.celrep.2014.10.032

Figure 1.

Figure 1

Modulation of GLP-1 Secretion in GLUTag Cells by Indole

(A) Cumulative GLP-1 secretion from GLUTag cells stimulated with 1 mM glucose, measured at different time points. Cells were incubated with and without 1 mM indole, and secretion was normalized to the 60 min, 1 mM glucose, time point (average value of 15 pg/ml) from the same experiment. Each data point was calculated by averaging over eight or more independent measurements. Significance was calculated at specific time points comparing the secretion at 1 mM glucose and the secretion at 1 mM glucose + 1 mM indole.

(B) Calculated rates of GLP-1 secretion over the time periods indicated, calculated from the data in (A). a.u., arbitrary units.

(C) GLP-1 secretion measured in response to different indole concentrations for an incubation time of 6 min in the presence of 1 mM glucose (measured from three independent measurements). To gain resolution the data were measured on a plate with high cell density (45 pg/ml GLP-1 secreted after 6 min in 1 mM glucose).

(D) GLP-1 secretion measured in response to different indole concentration for an incubation time of 240 min on a plate with high cell density (measured from three independent measurements). Data are presented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 by paired Student’s t test. The lines between points are drawn to guide the eye.