Table 1.
Source, dilution and pre-treatment of antibodies used
| Antibody | Dilution | Source/clone | Pretreatment/solution | Cut-off |
|---|---|---|---|---|
| Hormone receptor | ||||
| ERa | 1:200 | DAKO, ID5 | Microwave, citrate pH 6.0 | ≥1 % |
| DNA damage repair molecules | ||||
| PARP1 (Cleaved) | 1:1,000 | Abnova, A6.4.12 | Microwave, citrate pH 6.0 | >200 H-score |
| PARP1 (Non-cleaved) | 1:1,000 | BD Pharmingen, 7D3-6 | Microwave, citrate pH 6.0 | >10 H-score |
| RAD51 | 1:70 | Abcam, mouse polyclonal | Microwave, citrate pH 6.0 | |
| BRCA1 | 1:150 | Oncogene research, MS110 | Microwave, citrate pH 6.0 | >100 H-score |
| Ku70/Ku80 | 1:2,500 | Abcam, mouse monoclonal | Microwave, citrate pH 6.0 | >90 H-score |
| PIASγ | 1:50 | Abcam, anti E3 (ab61405) | Microwave, citrate pH 6.0 | >160 H-score |
| BRCA1 transcriptional suppressor molecule | ||||
| ID4 | 1:300 | Abcam, ab 20988- 100 | Microwave, citrate pH 6.0 | >12 H-score |
| EGFR family members | ||||
| EGFR | 1:30 | Zymed, 31G7 | Proteinase K | >10 % |
| c-erbB-2 (HER2) | 1:250 | DAKO, c-erbB-2 | None | 3+ (2+ with CISH) |
| Cell cycle, proliferation and apoptosis related proteins | ||||
| p53 | 1:50 | Novocstra, DO7 | Microwave, citrate pH 6.0 | >10 % |
| MDM2 | 1:50 | Novocastra, IB10 | Microwave, citrate pH 6.0 | ≥10 % |
| Ki-67/clone MIB1a | 1:100 | DAKO, M7240 | Microwave, citrate pH 6.0 | >10 % |
| Bcl2 | 1:100 | DAKO, 124 | Microwave, citrate pH 6.0 | >10 % |
| CHK1 | 1:150 | Abcam, rabbit polyclonal | Microwave, citrate pH 6.0 | >30 H-score |
| CK5/6 | 1:100 | Boehringer biochemica, D5/16134 | Microwave, citrate pH 6.0 | >10 % |
| CK14 | 1:100 | Novocastra, LL002 | Microwave, citrate pH 6.0 | >10 % |
| CK17 | 1:20 | Abcam, E3 | Microwave, citrate pH 6.0 | >10 % |
aPerformed on full face formalin fixed, paraffin embedded tissue sections