Table 1.
Primers used in qPCR to amplify four target regions
| Name | Sequence (5′–3′) | Target group | Amplicon size (bp) | Template DNA | Annealing term (°C) | References |
|---|---|---|---|---|---|---|
| Lact-F | AGCAGTAGGGA ATCTTCCA (19) | Lactobacillus | 341 | L. plantarum 299v | 61 | Walter et al. [19] |
| Lact-R | CACCGCTACAC ATGGAG (17) | Heilig et al. [20] | ||||
| Uni331-F | TCCTACGGGAG GCAGCAGT (19) | Total bacteria | 466 | L. plantarum 299v | 58 | Nadkarni et al. [22] |
| Uni797-R | GGACTACCAGG GTATCTAATCCTGTT (26) | |||||
| Ecol457-F | CATTGACGTTAC CCGCAGAAGAAGC (25) | Enterobacteriaceae | 195 | E.coli | 60 | Bartosch et al. [21]. |
| Ecol652-R | CTCTACGAGACT CAAGCTTGC (21) | |||||
| AMI-F | CAGCACGTGAA GGTGGGGGACC (20) | Akkermansia | 327 | A. municiphila | 60 | Collado et al. [23] |
| AM2-R | CCTTGCGGTTGG CTTCAGAT (20) | |||||
| DSV691-F | CCGTAGATATCT GGAGGAACATCAG | Desulfovibrio sp. | 135 | D. desulfuricans subsp. desulfuricans | 62 | Fite et al. [24] |
| DSV826-R | ACATCTAGCATC CATCGTTTACAGC |