Figure 7. Resveratrol enhanced mitochondrial membrane depolarisation induced by H₂O₂.

(ai) C2C12 myoblasts were exposed to 1000 µM H₂O₂ with or without RS preconditioning for 24 h as described in the text. Appropriate amounts of DMSO (DM pre-conditioning), followed by incubation with 1000 µM H₂O₂ (24 h), were used to ensure that the effects were resveratrol-specific. A positive control of mitochondrial depolarisation was obtained by two hours treatment with 5 µM of the apoptotic inducer staurosporine. The total % JC-1 green fluorescence cell population, including shift in depolarisation (gate UR+LR) was calculated. (a) representative images showing each condition and cell percentages in each gate from independent experiments. (aii) Percentage of depolarised cells (gate UR+LR) under different conditions. H₂O₂ with/without RS pre-conditioning induced mitochondrial depolarisation. Notably, with respect to H₂O₂ alone, RS pre-conditioning enhanced the effect of H₂O₂ toward the depolarised state (ai, LR panels), in a dose-dependent manner. DM+1000 µM H₂O₂ vs. CT+1000 µM H₂O₂ in none of the cases significant. Data are expressed as mean ± s.e.m. P-values calculated using a two-tailed Student's t-test. *: P = 0.01 vs NT (untreated); ¤: P<0.05 vs H₂O₂ 1000 µM. Each experiment was performed in quadruplicate.