Figure 3. Genome-wide conservation of antisense expression and splicing profiles.

(a) The joint distribution of the average antisense-to-total expression ratio (the number of reads mapped to the opposite strand as a fraction of the number of reads mapped to both strands) in pairs of orthologous protein-coding genes; cc=0.68. (b,c) Contour plots of the joint probability distribution of the average usage (ψ, per cent-spliced-in) of splice junctions (SJ; b), and of the standard deviation of SJ usage (c) in orthologous SJ pairs. Logistic transformation (logit) was used in a and b. SJ with constant complete inclusion or exclusion are not shown. ‘Alternative’ denotes SJ that are annotated alternative in both species.