Table 1.
miRNAs predicted to target the UTRs of selected drug metabolizing CYPs.
CYP Gene | Reference ID a | 3′-UTR length (bp) | Bioinformatic Programs b, c | Total no. of unique miRNAs d | Overlap e (%) | ||||
---|---|---|---|---|---|---|---|---|---|
Micro Cosm | miRanda | Target Scan | RNA22 | PITA | |||||
1A1 | NC_000015.8 (NM_000499) | 946 | 14 | 2 | 85 | 264 | 14 | 332 | 47 (12.4) |
1A2 | NC_000015.8 (NM_000761) | 1512 | 7 | g | 70 | 341 | 11 | 386 | 43 (10) |
1B1 | NC_000002.10 (NM_000104) | 3119 | 1 | 49 | 215 | 345 | 40 | 499 | 151 (23.2) |
2A6 | NC_000019.8 (NM_000762) | 257 | g | g | 18 | 37 | 2 | 54 | 3 (5.3) |
2B6 | NC_000019.8 (NM_000767) | 1569 | 5 | g | 125 | 347 | 27 | 416 | 88 (17.5) |
2C8 | NC_000010.9 (NM_000770) | 355 | 35 | g | 31 | 65 | 2 | 114 | 19 (14.3) |
2C9 | NC_000010.9 (NM_000771) | 362 | 46 | g | 34 | 77 | 9 | 129 | 37 (22.3) |
2C19 | L39102.1 | f | 57 | g | g | g | g | 57 | 0 |
2D6 | NC_000022.9 (NM_000106) | 74 | 33 | g | g | 4 | 4 | 40 | 1 (2.4) |
2E1 | NC_000010.9 (NM_000773) | 152 | 24 | g | 10 | 5 | g | 35 | 4 (10.3) |
3A4 | NC_000007.12 (NM_017460) | 1152 | 34 | 26 | 111 | 208 | 17 | 333 | 63 (15.9) |
3A5 | NC_000007.12 (NM_000777) | 110 | 12 | g | 11 | 14 | 1 | 32 | 6 (15.8) |
Reference sequence and the corresponding RefSeq Gene id from UCSC Genome browser in parenthesis.
PicTar predictions are not included in the table as the CYP genes do not appear to be a part of the program’s database.
Versions of the bioinformatic programs (retrieved on 07/12/2010): (A) MicroCosm Target uses miRNA Registry release 15.0, (B) for RNA22, we used miRNA Registry release 15.0, (C) MicroRNA.org and PITA use miRNA Registry release 11.0, and (D) TargetScan version 5.1 uses miRNA Registry release 10.1. Some of the predictions include multiple transcripts of the same gene.
Total number of unique miRNAs predicted by all the programs.
Total number of miRNAs predicted to target the genes by at least 2 programs.
No 3′-UTR sequence available in UCSC genome browser.
No results predicted for these CYPs by the corresponding bioinformatic program.