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. Author manuscript; available in PMC: 2015 May 17.
Published in final edited form as: Nat Commun. 2014 Nov 17;5:5475. doi: 10.1038/ncomms6475

Figure 4. Light-induced dimerization at centromeres.

Figure 4

Cells expressing CENPB–GFP-Haloenzyme and mCherry–eDHFR were treated with 20µM cTMP-Htag for 1h, then washed before imaging. (a) Cell-wide recruitment of mCherry–eDHFR to centromere-localized CENPB–GFP–Haloenzyme in response to a 2-s pulse of 387(±5.5)nm light. (b) Average mCherry–eDHFR centromere intensity at time points before and after uncaging, error bars represent s.d. (n=10 cells). (c) A single centromere (indicated by arrowhead in inset) was irradiated with a 405nm laser to induce mCherry–eDHFR recruitment. Insets show boxed regions in GFP (top row) and mCherry (middle row) and colour-merge (bottom row) from indicated time points. Scale bars, 5 or 1mm in insets. a.u., arbitrary unit.

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