(A) esiRNAs silence effectively in ESCs. Brightfield and GFP fluorescence images of ESCs bearing an EGFP transgene transfected with indicated esiRNAs. Images were obtained after 4 days of KD, except for Polr2A, which was imaged after 2 days.
(B) Western blotting of indicated proteins in ESCs knocked down as in (A). The asterisk indicates a background band in the Oct4 blot, which serves as a loading control.
(C) Examples of phenotypes from the esiRNA screen. ESCs were mock-transfected or transfected with the indicated esiRNAs and imaged after 4 days. Scale bars equal 140 μm.