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. 2015 Jan 13;137(2):568–571. doi: 10.1021/ja5107018

Figure 2.

Figure 2

(A) Excitation spectra of 2 in DPPC LUVs (solid) and DOPC LUVs (dotted) at 25 °C (blue) and 55 °C (red, λem = 600 nm). (B) Same for emission (λex = 420 nm). (C) Excitation spectra of 4 in DPPC LUVs (solid) and DOPC LUVs (dotted) at 25 °C (blue) and 55 °C (red, λem = 600 nm). (D) Time-resolved fluorescence decay of 2 (circles) and 1 (squares) in DPPC LUVs (empty) and DOPC LUVs (filled) at 25 °C. (E and F) Transcription of excitation shift to emission shift by FRET. (E) Excitation spectra of donor 3 (dashed, λem = 460 nm) and acceptor 2 (dotted, λem = 600 nm) in DPPC (blue) and DOPC (red). (F) Emission spectra of an equimolar mixture of donor 3 and acceptor 2 in DPPC (blue, solid) and DOPC (red, dashed, λex = 405 nm, blue arrow in E) with the following controls: Emission spectra of donor 3 (cyan, dashed) and acceptor 2 (blue, dotted) in DPPC, excitation spectrum of acceptor 2 in DPPC (gray, dotted) and DOPC (black, dotted), all at 25 °C.