Table 2.

Analysis of Auxotrophic Mutants

Mutant	Gene	Insertion site
15-04	ILV1	aa 268
31-36	ARG2	aa 441
37-26	SUL2	aa 388
42-37	LYS14	aa 755
47-40	MET3	aa 245
48-14	LYS12	aa 348
53-73	ARG8	aa 113
59-09	ARG7	aa 110
85-62	LYS9	aa 116
92-53	GDH1	aa 148
95-75	LYS5	aa 240

Shown are the genes disrupted for the 11 different loci disrupted in a total of 43 mutants (derived by homologous recombination) isolated by screening 9216 mutants. The position of the Tn7 insertion for each of the insertions is given. For multiple auxotrophic mutants derived from the same fosmid (and therefore predicted to be mutants in the same gene), at least two insertions were sequenced. In all cases, as expected, the same gene was disrupted but at a different site of insertion.