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. 2012 Jun 15;7(17):1304–1311. doi: 10.3969/j.issn.1673-5374.2012.17.004

Figure 4.

Figure 4

Changes in glutamate cysteine ligase catalytic subunit (GCLC) and glutamate cysteine ligase modulatory subunit (GCLM) mRNA expression in the rat cerebral cortex and hippocampus.

(A) RT-PCR analysis for mRNA expression of GCLM and GCLC in the cerebral cortex; (B, C) quantification of GCLM and GCLC mRNA expression in the cerebral cortex compared with the control (CON) group (adjusted as 1.0).

(D) Reverse transcription-PCR analysis for mRNA expression of GCLM and GCLC in the hippocampus; (E, F) quantification of GCLM and GCLC mRNA expression in the hippocampus compared with the control group (adjusted as 1.0).

GAPDH was used as an internal standard. aP < 0.05, bP < 0.01, vs. control group (one-way analysis of variance followed by Dunnett's t-test). The assay was performed three times and data were expressed as mean ± SD.