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. 2015 Jan 22;15:2. doi: 10.1186/s12876-015-0227-0

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© Kang et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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In situ hybridization for miR-21 and immunohistochemistry for E-cadherin and MTA1. (A) A representative 2 mm tumor tissue core from the colorectal cancer tissue microarray shows diffuse strong miR-21 expression in the stroma. (B) High-magnification image of insert in (A) shows that miR-21 signals are strong in the stromal cells of colorectal cancer but not in the tumor cells. Magnification x400. (C) Tumor cells show strong membranous expression of E-cadherin. Magnification x400. (D) Tumor cells show strong nuclear expression of MTA1. Magnification x400.