Table 2.

Wheat gene-specific primers for quantitative real-time RT PCR (RT-qPCR)

Gene	Abbreviation	GenBank	Primer Sequence
		Accession
ubiquitin	Ta-ubq	X56803	5’ggtgtctccggtatcctccaa 3’
			5’ tgctccacaccagcagaagt 3’
Polyamine biosynthesis
ornithine decarboxylase	Ta-odc	XP_003578555	5’ gctccaacttcaacggcttct 3’
			5’ cgaatggcgtgtgctacgta 3’
arginine decarboxylase	Ta-adc	EU236151	5’ gttgtatcgtgttactcatggtcgta 3’
			5’ gacgcatgggaaataaaaagatg 3’
s-adenosylmethionine	Hfr-samdc	HQ121401	5’ aggcaagctcgccaacct 3’
decarboxylase			5’ ggaatagcgacagcaaatcatg 3’
s-adenosylmethionine	Ta-sams	EMS47328	5’ cgtcatcggcggacctca 3’
synthetase			5’ ttggtcgggtccttgccagagaa 3’
spermidine synthase	Hfr-spds	HQ121400	5’ gcggtgttctttctaatctagctgaa 3’
			5’ gtgcacgccacccttgaata 3’
arginase	Ta-arg	CA598716	5’ gaagctgagcgcccaaga 3’
			5’ tttgttgcttcggtcctgact 3’
Ornithine biosynthesis
pyrroline-5-carboxylate	Ta-p5cs	AFO22914	5’ gcaccctcgaatttgttgatg 3’
synthetase			5’ acaatctgtgtgtgcacttccat 3’
ornithine aminotransferase	Ta-oat	AFO22915	5’ ggcacggaggcaaatgag 3
			5’ agtgaaataatgtcatgggaacca 3’
pyrroline-5-carboxylate	Ta-p5cr	AY880317	5’ ttcccctgcaggaactacca 3’
reductase			5’ gcatcttgttgtggcagcaa 3’
glutamate reductase	Ta-glr	BJ219898	5’ gcgtggtggatgaaagttcttat 3’
			5’ tgccggcaacttatgatgaa 3’
acetylornithinase	Ta-aor	BJ321340	5’ tctgcgaggaaggcttgaa 3’
			5’ agattacaggccaccccattc 3’