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. 2015 Jan 22;8:4. doi: 10.1186/s13068-014-0184-9

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© Muto et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Heterologous gene detection in Fistulifera solaris transformed with pSP-GK1/fcpB or pSP-GK2/fcpB. The nptII (A) and nptII-glycerol kinase (GK) gene fragments (B) were PCR-amplified. M, 1-kb marker; P, pSP-GK1/fcpB as the positive control; W, wild type; N, negative control transformed with pSP-NPT/H4; GK1, transformed with pSP-GK1/fcpB; GK2, transformed with pSP-GK2/fcpB.