Table 4.
Novel miRNA in the Diospyros kaki small RNA libraries constructed using fruits collected at 15 and 20 weeks after flowering (WAF)
| Names | Sequences | Transcriptome ID | Score of minimal free energy (MFE) | 15 WAF | 20 WAF | miRNA* | Star sequences | Fold change |
|---|---|---|---|---|---|---|---|---|
| dka-miRN01 | aacccgaaauucgagaaagac | MGB_c3657_6185 | 1.2 | 20 | 0 | No | ucguucgauuucgggucuggc | −8.36 |
| dka-miRN02 | aagaccugagaaugcaggaaagcuu | MGB_lrc11318_15117 | 1.8 | 10 | 0 | No | gcugcugcacuuaaggucggaa | −7.36 |
| dka-miRN03 | aagucuaugauaagauggcugagu | MGB_c832_1961 | 0.2 | 196 | 135 | No | gcaucuaguuauuguacaacuugc | −0.83 |
| dka-miRN04 | aaguggugcuuggaggaacuc | MGB_s80448_44080 | 1.7 | 0 | 15 | No | gauucucgagugccagaagg | 7.66 |
| dka-miRN05 | acggaguagaagccggccgagaau | MGB_s66037_35157 | 1.7 | 10 | 0 | No | caucguccggcuucuccaccgaga | −7.36 |
| dka-miRN06 | acuuuacaaauaaagcccgaggau | MGB_lrc56264_28052 | 0.1 | 0 | 10 | No | cuuugggcuuugauuaccaaggaa | 7.07 |
| dka-miRN07 | agggugcggguuugggacagcc | MGB_lrc64120_34018 | 2 | 2220 | 1935 | No | guguuugauccgcacccuuu | −0.49 |
| dka-miRN08 | aguacuugaggagaaggaaca | MGB_c17696_19188 | 0.8 | 14 | 0 | No | auugauuucuggaaguaguag | −7.84 |
| dka-miRN09 | caauaaauucuagaauuuuggagu | MGB_c35059_23284 | 4.9 | 324 | 175 | Yes | ccaagauuuagagacuuuaaagau | −1.18 |
| dka-miRN10 | cagaguacuacacuagcaacgcaua | MGB_c5218_8307 | 2 | 10 | 0 | No | uguguguuuguguugggcuuuggg | −7.36 |
| dka-miRN11 | ccuaaauuagugauggcuuagggc | MGB_c45699_25796 | 1.3 | 40 | 30 | No | ccuugagccgcuccugnunungagg | −0.70 |
| dka-miRN12 | auguaacagucacaugguagu | MGB_c66734_31898 | 1.8 | 0 | 20 | No | uaccaugugauugucacauga | 8.07 |
| dka-miRN13 | cggccgaaucguuggaugcgcc | MGB_c71082_39230 | 1.3 | 25 | 10 | Yes | ucuacauuauucggcuauagu | −1.61 |
| dka-miRN14 | cuacaugaauucuagacuucuaug | MGB_c80774_44313 | 1.2 | 12780 | 0 | Yes | uuugucuagaauucauauuuggau | −17.67 |
| dka-miRN15 | caugucaacuaaguuauggaaca | MGB_lrc74843_39335 | 8.5 | 350 | 0 | Yes | uuuccauauuguacuuguugg | −12.49 |
| dka-miRN16 | cccguagguuauaggguuggcaac | MGB_c1713_3178 | 2.2 | 0 | 142 | No | cuuuaaccugaacuuaccuuggcc | 10.90 |
| dka-miRN17 | cuccuaacucuugcccauuuau | MGB_lrc49089_29889 | 3.1 | 10 | 0 | No | aaaugggcaagaguuaggagag | −7.36 |
| dka-miRN18 | gagauaccagggaguagcagc | MGB_c8777_12556 | 2.7 | 10 | 0 | No | aaguagauccaucggauu | −7.36 |
| dka-miRN19 | gauucaagaaagcuguggaau | MGB_lrc36442_25847 | 2.2 | 1152 | 988 | Yes | uccacaggcuuucuugaacugc | −0.51 |
| dka-miRN20 | gggaauguacggaucuguaagua | MGB_c69353_37788 | 1.9 | 40 | 0 | No | cuugcgauuugugcuucguuu | −9.36 |
| dka-miRN21 | ggucaauggucagugacuaggagu | MGB_c36458_25861 | 1.6 | 95 | 0 | No | ucuaacgucaccaaacaguugaccc | −10.61 |
| dka-miRN22 | gugaauauugcagaccagcgg | MGB_lrc29703_21994 | 2.4 | 0 | 10 | No | ucuggucugcaauauucaccc | 7.07 |
| dka-miRN23 | ggagaugccagggagcagcgc | MGB_c6581_10173 | 2.7 | 0 | 211 | No | gaugcucccucguaucuccau | 11.46 |
| dka-miRN24 | guuggggauccauucgacuggggu | MGB_c8988_13431 | 1.7 | 0 | 10 | No | uucaguugggguuuuucgauug | 7.07 |
| dka-miRN25 | gguaugcauuccgcuguaggacc | MGB_c59771_28802 | 2.5 | 0 | 25 | No | ucuuacagcagaaugcauacugc | 8.39 |
| dka-miRN26 | guuugugaccaggggauuccc | MGB_c68424_33583 | 1.4 | 0 | 15 | No | uuaucacuacugggcccauaagucu | 7.66 |
| dka-miRN27 | uaaauccgagggaucuacuuu | MGB_c74194_41683 | 2.3 | 6404 | 5852 | Yes | aaguagauccaucggauu | −0.42 |
| dka-miRN28 | ucuacuuuauagaacaccggc | MGB_c43859_28278 | 1.6 | 1349 | 0 | No | uguuuucuauaaaguaaauc | 14.43 |
| dka-miRN29 | auagcgguccuacagcgguaugca | MGB_c17398_18816 | 2.2 | 0 | 8 | No | auuccgcuguagacccgugagcuc | 7.17 |
| dka-miRN30 | ugagaaccaugugaaucacu | MGB_lrc38668_26638 | 1.4 | 20 | 0 | No | ugauucacaugguucucgcc | −8.36 |
| dka-miRN31 | uaugcauaccgcuguaggacc | MGB_c17398_18804 | 2.2 | 98 | 0 | Nes | gucuacagcggaauguauacc | −10.65 |
| dka-miRN32 | ugggaaagcuggcuuucgagg | MGB_s83401_48752 | 1.7 | 10 | 0 | No | gagguggccaggauuuugca | −7.36 |
| dka-miRN33 | uuuauaaaguagaucucucgg | MGB_c33911_25155 | 1.7 | 148 | 0 | No | aagggaucuacuuuacugaac | −11.25 |