Table 1.

Primer sequences and PCR reaction conditions to amplify pfhrp2 , pfhrp3 and their respective flanking genes

Gene	Reaction	Primer name	Primer sequence	Annealing temperature, X (°C)	Expected amplicon size (bp)
PF3D7_0831900 (MAL7P1.230)	Primary	230 F1	5′ GAT ATC ATT AGA AAA CAA GAG CTT AG 3′	63	301
		230R	5′ TAT CCA ATC CTT CCT TTG CAA CAC C 3′
	Nested	230 F	5′ TAT GAA CGC AAT TTA AGT GAG GCA G 3′	65
		230R	5′ TAT CCA ATC CTT CCT TTG CAA CAC C 3′
PfHRP2 Exon 1–2, PF3D7_0831800	Primary	2E12F1	5′ GGT TTC CTT CTC AAA AAA TAA AG 3′	55	228
		2E12R1	5′ TCT ACA TGT GCT TGA GTT TCG 3′
	Nested	2E12F	5′ GTA TTA TCC GCT GCC GTT TTT GCC 3′	62
		2E12R	5′ CTA CAC AAG TTA TTA TTA AAT GCG GAA 3′
PF3D7_0831700 (MAL7P1.228)	Primary	228 F	5′ AGA CAA GCT ACC AAA GAT GCA GGT G 3′	60	198
		228R	5′ TAA ATG TGT ATC TCC TGA GGT AGC 3′
	Nested	228 F1	5′ CCA TTG CTG GTT TAA ATG TTT TAA G 3′	63
		228R	5′ TAA ATG TGT ATC TCC TGA GGT AGC 3′
PF3D7_1372100, (MAL13P1.485)	Primary	485 F	5′ TTG AGT GCA ATG ATG AGT GGA G 3′	60	241
		485R	5′ AAA TCA TTT CCT TTT ACA CTA GTG C 3′
	Nested	485 F1	5′ GTT ACT ACA TTA GTG ATG CAT TC 3′	59
		485R	5′ AAA TCA TTT CCT TTT ACA CTA GTG C 3′
PfHRP3 Exon 1–2, PF3D7_1372200	Primary	3E12F1	5′ GGT TTC CTT CTC AAA AAA TAA AA 3′	53	225
		3E12R1	5′ CCT GCA TGT GCT TGA CTT TA 3′
	Nested	3E12F	5′ ATA TTA TCG CTG CCG TTT TTG CT 3′	62
		3E12R	5′ CTA AAC AAG TTA TTG TTA AAT TCG GAG 3′
PF3D7_1372400 (MAL13P1.475)	Primary	475 F	5′ TTC ATG AGT AGA TGT CCT AGG AG 3′	55	212
		475R	5′ TCG TAC AAT TCA TCA TAC TCA CC 3′
	Nested	475 F	5′ TTC ATG AGT AGA TGT CCT AGG AG 3′	61
		475R1	5′ GGA TGT TTC GAC ATT TTC GTC G 3′

All reactions were conducted using the following reaction conditions: 95°C/5 min – [95°C/30 sec; X/30 sec; 68°C/30 sec] × 30 cycles – 68°C/5 min – 4°C/∞. The old PlasmoDB gene IDs are included in parentheses below the current IDs.