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. 2014 Dec 12;16:496. doi: 10.1186/s13058-014-0496-5

Figure 3.

Figure 3

p68 gene expression is controlled by both β-catenin and c-Myc. (a) HEK293T cells were transfected with small interfering RNAs (siRNAs) targeting either β-catenin or c-Myc. Scrambled siRNA-transfected cells were kept as control. After 24 h, cells were transfected with wnt3a and kept for another 24 h. Whole cell lysates (WCLs) were prepared and proteins were analysed by immunoblotting (IB) as shown in the figure. (b, c) β-catenin was knocked down in presence or absence of c-Myc overexpression by ectopic expression of myc-tagged c-Myc in H1299 cells. WCLs and total RNAs of 36 h post-transfected cells were analysed for p68 expression by IB and qRT-PCR. (d, e) 4T1 cells were transfected with siRNA targeting transcription factor 4 (TCF4), c-Myc, p68 or scrambled siRNA independently, and expression of respective proteins and mRNAs were analysed by IB and qRT-PCR after 36 h of transfection.