Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 12;16:496. doi: 10.1186/s13058-014-0496-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Guturi et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Wnt signaling-mediated p68 upregulation is important in the induction of EMT. (a) 4T1 cells were transfected with either β-catenin or transcription factor 4 (TCF4). After 36 h, lysates were prepared and analysed for epithelial to mesenchymal transition (EMT) marker proteins by immunoblotting (IB). (b) MDA-MB 231 and 4T1 cells were transfected with scrambled, tcf4 and p68 small interfering RNAs (siRNAs) independently. After 48 h, whole cell lysates (WCLs) were prepared and analysed for EMT marker proteins by IB. (c) Wnt3a-MCF7 and empty vector (EV)-MCF7 (control) stable cells were analysed for their anchorage-independent growth by formation of soft-agar colonies. Quantification of the number of colonies and error bars are represented in the figure. Standard deviation (SD) was calculated from three independent biological repeats. Statistical significance was analysed by Student’s t test (P <0.001) (top). Colony-forming ability of Wnt3a-MCF7 and EV-MCF7 cells were determined after individual knockdown of β-catenin, tcf4 and p68 using respective siRNAs as depicted in the figure (bottom). (d) Wnt3a-MCF7 and EV-MCF7 (control) stable cells were analysed for their invasiveness using matrigel invasion assay. Error bars are represented in the figure. SD was calculated from three independent biological repeats. Statistical significance was analysed by Student’s t test (P <0.001).