Figure 1.
Validation of differentially expressed microarray genes using quantitative real-time polymerase chain reaction (qPCR). To assess robustness of the microarray data, a selection of differentially expressed microarray genes (‘*’ for each probe set, where P <0.05) were contrasted with their qPCR counterparts (n = 6). Increases were observed in all in genes by qPCR, which corroborated the microarray analysis, though there was some variation in the extent of upregulation.