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. 2003 May 1;13(5):925–931. doi: 10.1101/gr.939903

Table 1.

Primer Design

SNP name	Type	Allele-specific primers	Label^a	Reverse primers	Melting temperature	dbSNP ID (ss#)
216 S2	G/C	`ACCAGGCCAGGCCGGCC`	Tam	`TGGCCCTGTGCAGGCTGAAA`	68	SS5112738
		`TGCTGCCTCTGCTCCCAGGC`	Fam	`GGGAACCGCAGGAGTAGGCTCA`
216 M+1	G/T	`AAGGTTCAGGGTGAGGGTTTCGT`	Tam	`NA (Linear Assay)`		SS5112737
		`AAGGTTCAGGGTGAGGGTTTCGC`	Fam
216 ST+4	T/G	`CAGCATTTGGGAACTTCAAGT`	Fam	`CAAATCACCTCTGTCACCCC`	59	SS5112741
		`GTCCACTCTCCTGCTTCTCC`	Rox	`CCACCCAGGTCACAGAGAAC`
216 ST+7	T/C	`TTTGACTGTCCCCATCCCATCT`	Fam	`GTCTGTGGGCAGAGGAGCAA`	64	SS5112744
		`TGACTGTCCCCATCCCATCC`	Rox
216 V-2	A/G	`ACTGAGTGAGGATGGGCTCT`	Tam	`TGCAGTTCAAGTTCCTGGAG`	59	SS5112757
		`CTGAGTGAGGATGGGCTCC`	Fam
216 S+1	T/A	`GTGGCCTCCCAGTCAAGCGT`	Tam	`GTCAAGGGTGAGGCAGCCAG`	60	SS5112740
		`TGGGGCAGGGATCCACCCCCT`	Fam	`CGAGCGCCTGCTTCCTGAGCC`

^a

(Tam) TAMRA; (Fam) 6FAM.