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. 2014 Jun 3;3(3):e000716. doi: 10.1161/JAHA.113.000716

Figure 2.

Figure 2.

Sarcomeric protein phosphorylation. A, cTnI total phosphorylation was determined by ProQ‐Diamond staining, while total protein content was determined by SYPRO‐Ruby staining (nDon=10, nPAH=10). B, MyBPC total phosphorylation was determined by ProQ staining, while total protein content was determined by SYPRO staining (nDon=10, nPAH=10). C, Typical example of donor and PAH samples ProQ and SYPRO staining and the relative location of specific sarcomeric protein. ProQ staining annotations—ov, ovalbumin; β, β‐casein; MyBPC, myosin binding protein C; cTnT, cardiac troponin T; cTnI, cardiac troponin I; MLC2, myosin light chain 2. SYPRO staining annotations—MHC, myosin heavy chain. Data presented as mean±SEM. PAH inidcates pulmonary arterial hypertension.