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. 2014 May 20;3(3):e000796. doi: 10.1161/JAHA.114.000796

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© 2014 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Figure 3. — Presence of p‐Shox2a in the SAN of mouse and human embryos. Immunohistochemical staining using anti‐S92P (A, E) or anti‐S110P (B, F) specific antibodies demonstrates the presence of p‐Shox2a in the SAN of E11.5 (A, B) and E13.5 (E, F) mouse embryo. Immunohistochemical staining shows the expression of Hcn4 in the SAN of E11.5 (C) and E13.5 (G) mouse embryo. D, Immunohistochemical staining using anti‐S92P antibody reveals the presence of p‐SHOX2a in the SAN of human embryo at 7‐week gestation. F, Western blotting using anti‐S92P antibody reveals the presence of p‐Shox2a in E11.5 mouse embryonic hearts. Red arrows point to the SAN region. Scale bar=20 μm for all panels. SAN indicates sinoatrial node.