Skip to main content
. 2014 Jun 25;3(3):e000854. doi: 10.1161/JAHA.114.000854

Figure 1.

Figure 1.

Myostatin expression was assessed by real‐time PCR. A, Myostsatin mRNA expression in different tissues of lean mice (n=6). B, Myostsatin mRNA expression in skeletal muclse of lean, lean myostatin−/−, db/db, and db/db myostatin−/− mice (n=8). A and B, Relative gene expression levels were quantified using the 2‐ΔΔCt approximation method. Gene expression was normalized twice to a control sample that was additionally normalized to GAPDH. Data are shown as mean±SEM. ***P<0.001, lean myostatin−/− versus lean or db/db myostatin−/− versus db/db. ##P<0.01, db/db versus lean or db/db myostatin−/− versus lean myostatin−/−. db/db myostatin−/− indicates mice lacking both myostatin and leptin receptor; db/db, obese leptin receptor‐deficient mice heterozygous for myostastin; lean myostatin−/−, myostatin‐null mice heterozygous for leptin receptors; lean, lean dual heterozygotes; PCR, polymerase chain reaction.