Figure 10.

Elevated oxidant load in the endothelium of obese db/db mice is reduced with increases in muscle mass. A, Superoxide scavenging by Tempol produced an improvement of acetylcholine‐induced dilation in obese mice. *P<0.05, in the absence of Tempol (−) versus in the presence of Tempol (+). B, Oxidized DNA marker 8‐OHG is elevated in the endothelium of obese db/db mice and reduced by deletion of myostatin. The data are given as the mean±SEM (A and B: n=6 to 8). 8‐OHG indicates 8‐hydroxyguanosine; DAPI, 4’,6‐diamidino‐2‐phenylindole; db/db myostatin^−/−, mice lacking both myostatin and leptin receptor; db/db, obese leptin receptor‐deficient mice heterozygous for myostastin; lean myostatin^−/−, myostatin‐null mice heterozygous for leptin receptors; lean, lean dual heterozygotes.