Table 2.

Primers Sequences That Were Used in Real‐Time PCR Procedure

Genes	Primer Sequences
GAPDH F	5′‐ACCCAGAAGACTGTGGATGG‐3′
GAPDH R	5′‐CACATTGGGGGTAGGAACAC‐3′
Myostatin F	5′‐CTGTAACCTTCCCAGGACCA‐3′
Myostatin R	5′‐TCTTTTGGGTGCGATAATCC‐3′
eNOS F	5′‐ACCCCCGGCGCTACGAAGAATG‐3′
eNOS R	5′‐GGTGGGCGCTGGGTGCTGAA‐3′
AcvRIIB F	5′‐TCAATTGCTACGACAGGCAG‐3′
AcvRIIB R	5′‐TGGCTCGTACGTGACTTCTG‐3′

eNOS indicates endothelial nitric oxide synthase; PCR, polymerase chain reaction.