Table 3.
Contents in Semisolid Culture for EPC‐CFA
| Company, Catalog No. | Final Concentration | |
|---|---|---|
| MethoCult™ SFBIT H4236 | STEMCELL Technologies, #04236 | |
| rh SCF | Peprotec, #300‐07 | 66.7 ng/mL |
| rh VEGF | Peprotec, #100‐20 | 33.3 ng/mL |
| rh basic FGF | Peprotec, #100‐18B | 33.3 ng/mL |
| rh EGF | Peprotec, #100‐15 | 33.3 ng/mL |
| rh IGF‐1 | Peprotec, #100‐11 | 33.3 ng/mL |
| rh IL‐3 | Peprotec, #200‐03 | 13.3 ng/mL |
| Heparin | Shimizu Pharmaceutical Co | 1.33 IU/mL |
| FBS | SAFC Biosciences, #12303 | 30% (vol/vol) |
EGF indicates epidermal growth factor; FGF, fibroblast growth factor; IGF, insulin‐like growth factor; IL, interleukin; rh, recombinant human; VEGF, vascular endothelial growth factor.