Table 7.

Human PCR Primers and Probes for qRT‐PCR in PBMNC and QQMNC

Gene	Forward Primer	Reverse Primer	TaqMan Probe (5′‐FAM, 3′‐BHQ)
VEGF‐A	5′‐CCCAGGAGACCT 5′‐GGTTGTGT‐3′	5′‐TGGATCCTGCCCTGT CTCTCT‐3′	5′‐AGTGGTTGACCTTCC TCCATCCCC‐3′
VEGF‐B	5′‐AGGTGACACATG GCTTTTCAG A‐3′	5′‐GTTCCCCCACTGGGA TATAGC‐3′	5′‐TCAGCAGGGTGACTT GCCTCAGA‐3′
Ang‐1	5′‐AAGCTACTGGGC CTCCTCTCA‐3′	5′‐CCATTAAGGCAT AGTGGATCAAGTC‐3′	5′‐AAAAGAGACAGTTGT TGGCAAGGTAGCAA‐3′
Ang‐2	5′‐GCAGGGAGTGG TGAGACAGTT‐3′	5′‐TGCAGGTGCTATGGT CTTTAGAAT‐3′	5′‐ACGGCTCCTCAGAAA TCCAGTGACC‐3′
IGF‐1	5′‐GCCCAAAATGCA CTGATGTAAA‐3′	5′‐AGTGACTTTGCTATGAGTTGGTGAGT‐3′	5′‐CTCTAAAATCCCTTT CAAGCCACCCAT‐3′
Leptin	5′‐TCACTAGATGGCGAGCATCCT‐3′	5′‐CACGCTCAGCTAACTTTTGTGTTT‐3′	5′‐CCAACATGGTGAAACCCCGTCTCTAC‐3′
IL‐8	5′‐TTTGATACTCCCAGTCTTGTCATTG‐3′	5′‐CAAGTTTCAACCAGCAAGAAATTACT‐3′	5′‐TTAGAACTATTAAAACAGCCAAAACTCCACA‐3′
IL‐10	5′‐GCCTGACCACGCTTTCTAGCT‐3′	5′‐CCAAGCCCAGAGACAAGATAAATT‐3′	5′‐TTGAGCTGTTTTCCCTGACCTCCC‐3′
IL‐1 β	5′‐CGGCCACATTTGGTTCTAAGA‐3′	5′‐AGGGAAGCGGTTGCTCATC‐3′	5′‐ACCCTCTGTCATTCGCTCCCACA‐3′
TGF‐β	5′‐CCCTGCCCCTACATTTGGA‐3′	5′‐CCGGGTTATGCTGGTTGTACA‐3′	5′‐TGGACACGCAGTACAGCAAGGTCCT‐3′
TNF‐α	5′‐GAGACCAGGGAGCCTTTGGT‐3′	5′‐TGTGTCAATTTCTAGGTGAGGTCTTC‐3′	5′‐CTGGCCAGAATGCTGCAGGACTT‐3′
MMP‐2	5′‐GGTTCCCCTGTTCACTCTACTTAGC‐3′	5′‐CGGCTTGGTTTTCCTCCAT‐3′	5′‐TGTCCCTACCGAGTCTCTTCTCCACTG‐3′
MMP‐9	5′‐CCCGGAGTGAGTTGAACCA‐3′	5′‐AGGGCACTGCAGGATGTCA‐3′	5′‐TGGACCAAGTGGGCTACGTGACCT‐3′
GAPDH	5′‐GGTGGTCTCCTCTGACTTCAACA‐3′	5′‐GTGGTCGTTGAGGGCAATG‐3′	5′‐ACACCCACTCCTCCACCTTTGACG‐3′

Ang indicates angiopoietin; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; IGF, insulin‐like growth factor; IL, interleukin; MMP, matrix metalloproteinase; PBMNCs, peripheral blood mononuclear cells; QQMNCs, quality and quantity control culture of mononuclear cells; qRT‐PCR, quantitative real‐time polymerase chain reaction; TGF, transforming growth factor; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor.