Table 2.

CaM Mutants Do Not Exhibit Major Biophysical Effects on Peak I_Na

	Voltage‐Dependence of Activation			Steady‐State Availability			Recovery From Inactivation
	V1/2 (mV)	k (mV)	n	V1/2 (mV)	k (mV)	n	τ (ms)	n
Biophysical parameters
NaV1.5+WT CaM	−45.0±2.1	6.6±0.5	9	−102.9±3.2	−8.4±0.2	10	10.9±1.3	6
NaV1.5+CaM‐D130G	−43.1±2.3	6.6±0.5	11	−98.5±1.7	−8.1±0.1	10	8.8±1.1	8
NaV1.5+CaM‐D96V	−42.6±1.1	7.8±0.3	10	−105.2±1.0	−8.7±0.4	13	11.2±0.5	11
NaV1.5+CaM‐F142L	−41.8±2.0	6.7±0.8	10	−96.4±2.2	−8.5±0.4	11	8.7±0.6	10
Fetal NaV1.5+WT CaM	−38.4±1.7	7.5±0.5	11	−103.3±2.2	−9.7±1.0	9	9.1±0.8	10
Fetal NaV1.5+CaM‐D130G	−41.0±1.7	7.2±0.5	10	−98.6±1.2	−8.9±0.3	8	11.0±1.3	9
Fetal NaV1.5+CaM‐D96V	−35.2±2.1*	9.4±0.4*	9	−99.1±1.4	−13.1±2.3**	9	12.1±1.6	7
Fetal NaV1.5+CaM‐F142L	−35.4±1.7*	8.3±0.6	6	−96.3±1.7	−8.4±0.3	7	8.9±1.4	6

All recordings were performed with high intracellular calcium concentration. CaM indicates calmodulin; WT, wild type.

^*P<0.05 compared to Na_V1.5+WT CaM.

^*P<0.05 compared to fetal Na_V1.5+WT CaM.

^*P<0.005 compared to Na_V1.5+WT CaM.