Figure 5. Titration of NPN fluorescence emission with MOP.

( A) NPN (2.5 µM) was added to a solution of AaegOBP1 (0.35 µM) in ammonium acetate buffer, pH 7. ( B) NPN (2.5 µM) was added to ammonium acetate buffer, pH 7. In both cases, increasing aliquots of MOP were added and emission spectra were recorded.