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. 2015 Jan 12;35(1):353–363. doi: 10.1159/000369701

Fig. 1.

Fig. 1

PM treatment decreased murine circulating EPC level with increased EPC apoptosis. A. After exposure of C57BL/6 mice with PM or PBS through intranasal distillation for 1 month, the blood cells were collected after red blood cell lysis and stained with CD34-AF700 and CD133-PE antibody for EPC (CD34+/CD133+) measurement. The EPC level was significantly decreased in C57BL/6 mice with PM exposure compared to the PBS control. The annexin V (B) and PI (C) were used to incubate the blood cells for apoptosis analysis. Both early (B) and late (C) apoptotic rate of blood cells in the mice with PM exposure was significantly increased as compared to the PBS control group. WT+PBS: C57BL/6 mice with PBS treatment; WT+ PM: C57BL/6 mice with PM exposure. * WT+ PM vs WT+PBS, P<0.01, n=8.