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. 2014 Dec 9;16(12):1059–1069. doi: 10.1016/j.neo.2014.09.011

Figure 2.

Figure 2

ZBTB4 decreases expression of Sp transcription factors and EZH2 in breast cancer cells. (A) MCF-7 and MDA-MB-231 cells were transfected with ZBTB4 expression plasmid and decreased protein expression was determined by western blots of whole cell lysates. (B) The effects of ZBTB4 overexpression of levels of EZH2, Sp1, Sp3 and Sp4 mRNA levels were determined in MDA-M-231 and MCF-7 cells by real time PCR. (C) Luciferase activity was determined in breast cancer cells transfected with ZBTB4 expression plasmid and pEZH2, a construct containing the − 1046 to + 48 region of the EZH2 promoter.