|
| ||
| Component | Amount per reaction | Final |
|
| ||
| f88-4 vector | Depends on concentration | 50 μg |
| OR: fUSE5 vector | Depends on concentration | 50 μg |
| XbaI endonuclease | 0.5 μl | 5U |
| BamHI endonuclease | 0.5 μl | 5U |
| 10x endonuclease buffer Y | 5.0 μl | 1x |
| Milli-Q water | Up to 50 μl | |
|
| ||
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