Table 2. Different sample preparation techniques yield significant albumin removal with different total protein spots.
For all four sample types, antibody-based affinity chromatography proved to be the best technique for albumin removal and increased protein spots found in a 2D gel. For (A) mouse plasma/serum and (B) rat serum, TCA/acetone method provides the second most albumin removal and yields the second most 2-D gel spots.
| A | ||||||||
|---|---|---|---|---|---|---|---|---|
| Species | Mouse | |||||||
| Sample Type | Plasma | Serum | ||||||
| Albumin Removal Method | SwellGel | Protea Prep | TCA acetone | MARS Ms-3 | SwellGel | Protea Prep | TCA acetone | MARS Ms-3 |
| Albumin Removed (SDS-PAGE) | 51% | 26% | 39% | 68% | 39% | 23% | 33% | 73% |
| Total 2-D Spots Detected | 876 | 851 | 965 | 1352 | 974 | 1025 | 1083 | 1311 |
| B | ||||||||
|---|---|---|---|---|---|---|---|---|
| Species | Rat | |||||||
| Sample Type | Plasma | Serum | ||||||
| Albumin Removal Method | SwellGel | Protea Prep | TCA acetone | MARS Ms-3 | SwellGel | Protea Prep | TCA acetone | MARS Ms-3 |
| Albumin Removed (SDS-PAGE) | 40% | 24% | 32% | 56% | 34% | 23% | 36% | 49% |
| Total 2-D Spots Detected | 1008 | 943 | 1157 | 1285 | 1069 | 1069 | 1155 | 1263 |