Figure 7.
Effects of Tat and gp120 on endonuclease G (endo G) in the cytoplasm at 4 and 72 h following continuous treatment. HIV-1 Tat1-72 (100 nM) increased cytoplasmic levels of in striatal neurons at 4 hours in vitro (*P < 0.05 versus vehicle-treated control cultures), while gp120 (500 pM) failed to increase endo G levels in the cytoplasm (Cyto) at 4 h or 72 h. Although cytoplasmic changes in endo G are shown (B,C), mitochondrial (Mito) levels were also assessed and varied inversely to cytoplasmic levels in control and Tat-treated neurons as expected (A). The results show the mean ± SEM of n = 4 experiments.