Table 1.
Effect of sugar osmolytes on the refolding of denatured BlTreA at 25°C.
| Osmolyte concentration (M) | Sorbitol | Sucrose | Trehalose | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| I | II | III | IV | I | II | III | IV | I | II | III | IV | |
| 0.00 | 100 | 513.5 | 330.7 | 4.0 | 100 | 513.5 | 330.7 | 4.0 | 100 | 513.5 | 330.7 | 4.0 |
| 0.30 | 50.3 | 374.9 | 333.5 | 35.3 | 48.8 | 372.6 | 332.7 | 31.0 | 49.30 | 369.6 | 334.2 | 33.3 |
| 0.75 | 61.5 | 416.3 | 331.5 | 27.1 | 55.5 | 357.4 | 333.7 | 25.5 | 51.60 | 380.8 | 333.9 | 30.0 |
| 1.00 | 57.6 | 417.2 | 332.1 | 25.9 | 54.5 | 357.9 | 332.6 | 25.5 | 47.88 | 377.6 | 334.1 | 30.7 |
| 1.25 | 51.8 | 406.7 | 332.3 | 26.8 | 49.4 | 361.2 | 332.9 | 27.1 | 33.10 | 384.7 | 333.8 | 35.3 |
I: final refolding yield of BlTreA; II and III (nm): maximum intensity and peak position of the intrinsic fluorescence emission spectra of the refolded BlTreA in the presence of sugar osmolytes; IV: ANS fluorescence intensity of the refolded BlTreA in the presence of sugar osmolytes.