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. 2015 Jan 13;2015:475935. doi: 10.1155/2015/475935

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Copyright © 2015 Vijayalaxmi Gupta et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Morphological analysis of mouse testis after different postfixative manipulations. Sections of adult mouse testis were stained with HE (all at 60x; magnification bar is 40 μm). (a) Control; (b) slow rehydration-dehydration stepwise replacement of ETOH-PBS-ETOH; (c) slow Re-ETOH only stepwise replacement; (d) rapid ETOH-PBS-ETOH transition. Sertoli cell (Se), spermatogonia (Sp), spermatocytes (Sc), spermatids (Sd), and Leydig cell (L). Black arrows—abnormal open spaces in seminiferous epithelium; yellow arrows—abnormal wavy and thinner basement membrane.