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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Metallomics. 2014 Jul 24;6(10):1816–1823. doi: 10.1039/c4mt00168k

Figure 2. Acute Mn exposure activates the mitochondrial unfolded protein response.

Figure 2

A, B, Fluorescent photomicrographs of day 1 adult hermaphrodite worms expressing phsp-6::GFP, a transcriptional reporter for the mitochondrial UPR, after 24 hour treatment with water (Ctrl) or 20 mM MnCl2. C, Immunoblots of protein extracts from day 1 adult animals expressing phsp-6::GFP after 24 hour treatment with water (Ctrl), 10 mM, or 20 mM MnCl2. D, Quantification of GFP intensity from phsp-6::GFP immunoblots in arbitrary units. (**=p<0.01, Student's t-test) E, Immunoblots of protein extracts from day 1 adult animals expressing phsp-6::GFP after 24 hour treatment with water (Ctrl), 20 mM MgCl2, or 20 mM MnCl2. F, Immunoblots of protein extracts from day 1 adult animals expressing phsp-16.2::GFP, a transcriptional reporter for heat stress, after 24 hour treatment with water (Ctrl), 20 mM MnCl2, or a 1 hour heat stress at 33°C.