A, Quantification of number of day 1 animals that remained alive after a 24 hour treatment with water (Ctrl), 20 mM ChCl, or 20 mM MnCl2. All animals remained alive in Ctrl and ChCl-treated animals, while only ∼26% survive MnCl2 treatment (**=p<0.005, Student's Test). B, Quantification of body length of day 1 animals after 24 hour treatment with water (Ctrl), 20 mM ChCl, or 20 mM MnCl2. (**p=<0.0001, Student's t-test) C-E, Top panel, fluorescent photomicrographs of day 1 adults worms expressing 40 polyglutamines tagged to YFP in their body wall muscle (AM141 [rmIs133(P(unc-54) Q40::YFP)]) after 24 hour exposure to water (Ctrl), 20 mM ChCl, or 20 mM MnCl2. Bottom panel, bright field photomicrographs from top panels.