Fig. 2.

Cytokine gene expression among activated PBMCs. A, IL-2 gene expression levels in PBMCs from normal and MF/CTCL patients stimulated by PMA/A23187 versus anti-CD3/anti-CD28 antibodies. Quantitative RT-PCR was performed on PBMCs from normal (n = 3), early MF/CTCL (n = 2), and late MF/CTCL+B (n = 2) patients that were stimulated by PMA/A23187 or anti-CD3/anti-CD28 antibodies for the indicated time and analyzed for IL-2 gene expression. The relative level of cytokine gene expression was normalized to β2M level of gene expression and plotted to the unstimulated level of gene expression for the respective patient samples for IL-2. Points, mean; bars, SE. B–D, Cytokine gene expression among activated PBMCs from early and late MF/CTCL+B and MF/CTCL−B patients compared with normal and psoriasis patients. (B) Quantitative RT-PCR was performed on PBMCs from normal (n = 8), psoriasis (n = 6), early MF/CTCL (n = 8), and late MF/CTCL+B (n = 4) and late MF/CTCL−B (n = 3) patients that were stimulated for the indicated time and analyzed for (B), IL-2 (C), IL-4 (D) and IFN-γ gene expression. The relative level of cytokine gene expression was normalized to β2M level of gene expression and plotted to the unstimulated level of gene expression for normal PBMCs for IL-2, IL-4, and IFN-γ. Points, mean; bars, SE. Data points that included values that were computed from linear regression analysis are denoted with smaller markers.