Figure 2.
Overview of the study design. Beginning at approximately postnatal day 45 (P45), mice were administered 1 mg/kg AgNPs through tail vein injection 5 times, once every 3 days (corresponding to days 0, 3, 6, 9, and 12 post-exposure). Per post-exposure time point assessed (15, 60, and 120 days post-initial exposure), six control and six treated mice were used for isolation of blood, testes, and sperm. Meanwhile, a separate group of six control and six treated mice were used for breeding assessment. The numbers on the bottom right corner of the black boxes indicate a group of mice.