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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Reprod Toxicol. 2014 Jan 18;45:59–70. doi: 10.1016/j.reprotox.2014.01.006

Table I.

Sperm parameters as established by computer-assisted sperm analysis.

Day 15 Day 60 Day 120

Parameter Control (mean ± SEM) AgNP (mean ± SEM) Control (mean ± SEM) AgNP (mean ± SEM) Control (mean ± SEM) AgNP (mean ± SEM)
Smoothed path velocity (VAP) (μm/s) 115.68±9.04 118.42±6.18 134.93±6.89 109.38±9.06 117.43±4.39 116.6±3.84
Straight line velocity (VSL) (μm/s) 87.38±8.76 90.08±5.83 97.23±6.50 72.88±8.02 80.03±2.98 81.48±2.85
Track velocity (VCL) (μm/s) 208.14±13.12 219.28±8.55 240.23±9.69 202.83±12.98 223.52±5.46 221.28±4.83
Amplitude of lateral head displacement (ALH) (μm) 11.1±0.53 10.92±0.37 13.33±0.35 11.9±0.56 13.17±0.31 13±0.27
Beat cross frequency (BCF) (Hz) 29.74±0.64 32.86±1.70 25.83±0.73 28.28±1.06 28.58±1.19 28.47±1.32
Straightness (STR) (VSL/VAP) 71.2±1.77 70.8±1.46 69.25±1.25 63.5±2.40 65.33±0.56 66.83±0.91
Linearity (LIN) (VSL/VCL) 42.4±1.29 41.2±1.24 40.5±1.32 36.25±2.17 35.83±0.65 37±0.89
Elongation/Head shape (ratio of minor to major axis of sperm head) 43±0.63 42.8±0.86 42.25±0.75 46.25±1.84 43.67±0.42 42.67±0.80
Total (millions/ml) 16.22±2.16 11.62±1.62 28.63±1.89 36.43±4.21 35.32±2.35 31.17±4.00
Motile (%) 77.4±3.64 74.2±4.75 85.5±2.87 87.5±1.55 83.17±1.49 81.17±1.78
Progressive (%) 53.8±3.26 49.0±5.37 62.0±3.19 54.0±3.70 52.67±1.12 52.5±2.33
Rapid (%) 67.8±3.68 63.4±5.22 77.5±3.01 76.5±3.01 71.33±1.38 68.67±2.25
Slow (%) 1.6±0.24 2.2±0.58 1.25±0.25 1.5±0.29 1.33±0.21 1.83±0.17
Static (%) 20.8±3.57 23.6±4.35 13.5±2.87 11.25±1.44 15.3±1.52 17.17±1.70