Figure 4.

Uptake and distribution of ⁵⁵Fe in rice seedlings.

Two-week-rice seedlings were treated either with 5μM of ⁵⁵Fe-DMA or ⁵⁵Fe-EDTA for 4 h and were subjected to autoradiography. (a, b) Autoradiographs of rice seedlings treated with ⁵⁵Fe-DMA or ⁵⁵Fe-EDTA at pH 5.8 (a) and 8.0 (b).

(c, d) Photographs of rice seedlings used for autoradiographic analysis shown in panels (a) and (b), respectively. Scale bars = 4 cm.

(e, f) Quantification of ⁵⁵Fe signal intensity in rice shoots using the BAS-5000 system at pH 5.8 (e) and pH 8.0 (f). Control, no chelator added. All treatments contained equal amounts of Fe. Values shown are means ± standard deviation (SD) (n = 5).