Table 1. X-ray data collection and refinement statistics.
| TAF8–10Native | TAF8–10S-SAD | Importin α1/TAF8–NLSNative | |
|---|---|---|---|
| Data collection | |||
| Space group | P3121 | P3121 | P212121 |
| Cell dimensions | |||
| a, b, c (Å) | 51.32, 51.32, 144.40 | 51.30, 51.30, 144.70 | 54.27, 77.72, 128.57 |
| α, β, γ (°) | 90, 90, 120 | 90, 90, 120 | 90, 90, 90 |
| Wavelength | 0.98011 | 1.90745 | 0.93340 |
| Resolution (Å) | 44.44–1.91 (1.98–1.91)* | 48.23–2.61 | 49.54–1.75 (1.81–1.75) |
| Rmerge | 2.92 (87.98) | 2.00 | 5.00 (77.45) |
| I/σI | 23.4 (1.68) | 48.43 | 19.18 (2.03) |
| Completeness (%) | 93.91 (58.53) | 100.00 | 99.60 (99.14) |
| Redundancy | 4.80 (4.30) | 20.91 | 4.60 (4.60) |
| Refinement | |||
| Resolution (Å) | 44.44–1.91 | 49.54–1.75 | |
| No. of reflections | 16,793 (1,630) | 12,943 | 55,423 (5,436) |
| Rwork/Rfree | 20.5 (33.1)/23.7 (35.5) | 15.3 (23.9)/18.0 (27.2) | |
| No. of atoms | 1,474 | 3,877 | |
| Protein | 1,404 | 3,366 | |
| Ligand/ion | 7 | 48 | |
| Water | 63 | 463 | |
| B-factors | |||
| Protein | 58.2 | 30.9 | |
| Ligand/ion | 59.9 | 59.6 | |
| Water | 51.9 | 44.8 | |
| R.m.s. deviations | |||
| Bond lengths (Å) | 0.003 | 0.008 | |
| Bond angles (°) | 0.681 | 1.158 | |
R.m.s., root mean squared.
*Values in parentheses are for highest-resolution shell.