Fig 2.

Isothermal titration calorimetry data for Zn²⁺ binding to human histidine-rich glycoprotein (HRG). Here, 55 injections of 5 μL of 150 μm ZnCl₂ were delivered to samples of 10 μm HRG in buffer containing 50 mm Tris and 140 mm NaCl (pH 7.4) over a period of 10 s with an adequate interval (240 s) between injections to allow complete equilibration. The total Zn²⁺ concentration at the end of the experiment was 24.6 μm. Raw data are shown in Fig. S5.