Fig 6.

Speciation modeling analysis of plasma Zn²⁺. (A) Occupation of human serum albumin (HSA) site A in the presence of 0–5 mol. eq. myristate. (B) Proportion of Zn²⁺ bound to HRG in the presence of 0–5 mol. eq. myristate. Values for the stoichiometric factor for site A were extracted from several fits (Table S2) and averaged. For the determination of HRG loading with Zn²⁺ the conditional constants from Table 1 were used to model the distribution of Zn²⁺ (15 μm) in the presence of HSA (620 μm) and HRG (1 or 2 μm). Full speciation data are reported in Table S3. Errors correspond in each case to 1σ. (C) Reaction scheme showing the proposed effects that elevated FFA levels would have in relation to modulation of HRG–heparin interactions and destabilization of the thrombin-antithrombin III (ATIII) complex. Myr, myristate.