Fig. 2.

Cell-cycle timing probed by consensus localization patterns. To demonstrate the ability of consensus localization patterns to determine cell-cycle timing, we analyze the localization patterns of proteins in two processes where the cell-cycle ordering of protein localization is already known. Consensus location patterns are represented as kymographs.

A. SeqA and MukB are proteins implicated in chromosome replication and segregation. Consistent with previously reported results, the kymographs show MukB arriving at the quarter-cell position prior to SeqA.

B. FtsZ, MinD and SlmA are proteins implicated in cytokinesis. Both MinD and SlmA are known to inhibit FtsZ-dependent Z-ring formation. The kymographs show significant FtsZ localization at midcell only after MinD and SlmA are depleted from midcell, consistent with the known mechanism of z-ring regulation.