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. 2015 Jan 28;10(1):e0116575. doi: 10.1371/journal.pone.0116575

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© 2015 Yost et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Box plots (top) and difference plots (bottom) show data from primary human naïve and memory CD4⁺ T cells isolated from the blood of 30 healthy donors and stimulated for three days with plate-bound anti-CD3 and soluble anti-CD28 in conditions promoting TH1 (A) or TH2 (B) differentiation. IL-2 mRNA levels were determined by qPCR. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.