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. 2015 Jan 28;10(1):e0116575. doi: 10.1371/journal.pone.0116575

Figure 9. Potentiation of IL-2 mRNA increases requires continuous Gβγ inhibition during prolonged TCR stimulation.

Figure 9

(A) Gallein had minimal or no effect on IL-2 mRNA levels when added after the first day of TCR stimulation. Jurkat cells were stimulated with plate-bound anti-CD3 and soluble anti-CD28 antibodies for three days. Gallein or fluorescein was added from the beginning of TCR stimulation (0 days) or at the indicated times afterwards. IL-2 mRNA levels were determined by qPCR after three days of TCR stimulation. Data represent means ± SE from 5 experiments and were normalized to the value of the untreated control. n.s., not significant; *, p < 0.05; **, p < 0.01. (B) Treatment with gallein for only the first day of a three-day TCR stimulation was not sufficient to potentiate TCR-stimulated IL-2 mRNA levels. Jurkat cells were stimulated with plate-bound anti-CD3 and soluble anti-CD28 antibodies for three days in the absence of gallein (none), or in the presence of gallein for the full three days or only the first day. In all cases, the media was changed after the first day and replaced with media that contained anti-CD28 and either gallein (three days), or no gallein (none and first day). Data represent the means ± SE from 4 experiments and were normalized to the untreated control. *, p < 0.05.