Figure 1. Schematic representation of the model system.

The library preparation consists of six steps, beginning by extending randomized synthesized oligonucleotides (1), digesting the extended constructs (2), enriching the constructs and removing cleaved products (3), ligating sequencing adapters to the cleaved fragments (4), denaturing single stranded DNA from beads (5), and amplifying with PCR for sequencing (6).