Table 2. Distribution of total slippage detected of the restriction enzymes.
| Enzyme | Expected distance (bp) | Total slippage* | Isoschizomer | Organism |
|---|---|---|---|---|
| BseRI | 10 | 1.1% | - | Bacillus species R |
| AcuI | 16 | 1.1% | Eco57I | Acinetobacter calcoaceticus |
| BbvI | 12 | 1.3% | - | Bacillus brevis |
| BpmI | 16 | 1.5% | GsuI | Bacillus pumilus |
| FokI | 13 | 1.7% | - | Flavobacterium okeanokoites |
| GsuI | 16 | 5.1% | BpmI | Gluconobacter suboxydans H-15T |
| BsgI | 16 | 5.7% | - | Bacillus spaericus GC subgroup |
| Eco57I | 16 | 7.2% | AcuI | Escherichia coli RFL57 |
| Eco57MI(G) | 16 | 10.7% | - | Escherichia coli RFL57M |
| SmuI | 6 | 12.4% | FauI | Sphingobacterium multivorum RFL21 |
| Eco57MI(A) | 16 | 13.1% | - | Escherichia coli RFL57M |
| FauI | 6 | 15.2% | SmuI | Flavobacterium aquatile |
| EcoP15I | 27 | 25.8% | - | Escherichia coli P15 |
| BpuEI | 16 | 41.4% | - | Bacillus pumilus 2187a |
| MmeI | 20 | 53.6% | - | Methylophilus methylotrophus |
*Slippage is defined as a cleaving 1 or 2 bases up- or downstream of the expected site of cleavage.